Regulatory

Part:BBa_K1401007:Design

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-10-09)


Tandem promoter pTet-pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 159
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 141


Design Notes

We looked through the literature and decided to attach the two promoters without any extra sequence between them


Source

This was formed using BBa_R0040 and BBa_I13453

References